In the present study, it was aimed to find the role of calcium on the maturation of mouse follicular oocytes as well as for the role of calcium inhibitors, Ni2+ and La3+.
Mouse follicular oocytes were cultivated in different media at 37℃, in 100% humidified CO2 incubator for 3 and 17 hrs.
@ES The results were as follows;
@EN 1. There was no differences in GVBD between the control and experimental groups during the 3 hr culture.
2. Mouse oocytes were matured to higher rate in MHBS rather than HTF for 17 hr culture.
3. Maturation rate was significantly lower in Ca2+ -free and Ca2+ 0.4mM which were tested, compared to other calcium concentration used in the present study.
4. Calcium inhibitor, Ni2+, it showed highest degeneration rate at all calcium concentrations and additionally in Ni2+ 100μM treated group next. Maturation rate was significantly decrease as the Ca2+ inhibitor concentration increased.
5. In all Lanthanum treated groups of calcium-free, degeneration were significantly high treated groups at 0.4mM Ca2+ concentrations degeneration rates of all group were significantly lower than t hat of the control but maturation rates were not
significantly different in any group. In lanthanum 10μM treated group at 0.4mM and 0.8mM calcium concentration, its maturation rate was significantly higher than that of the control. Maturation rates of all groups of lanthanum treated at 1.71mM
calcium
concentration were not significantly different among groups.
6. In the calcium treated group (0.4mM-1.7mM), the presence of phosphate does not seem to be needed for oocyte maturation. However, the presence of phosphate at Ca2+ 0.8mM only seems to stimulated maturation.
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